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中华肾病研究电子杂志 ›› 2013, Vol. 02 ›› Issue (01) : 38 -41. doi: 10.3877/cma.j.issn.2095-3216.2013.01.008

论著

采用决策树方法建立基于UF-1000i尿液分析仪镜检规则的研究
曹源1, 王延群1, 孙子涵1, 孙黎1, 韩凝1, 杜秀敏1, 孔立新1, 綦瑞霞2, 胡成进1,()   
  1. 1.250031 山东 济南,济南军区总医院实验诊断科
    2.250031 山东 济南,山东省军区干休二所
  • 出版日期:2013-02-15
  • 通信作者: 胡成进

Construction of microscopy review rules based on UF-1000i using decision tree approach

Yuan CAO1, Yan-qun WANG1, Zi-han SUN1, Li SUN1, Ning HAN1, Xiu-min DU1, Li-xin KONG1, Ruixia QI1, Cheng-jin HU,1()   

  1. 1.Department of Laboratory Medicine, Jinan Military General Hospital, Jinan,Shandong 250031, China
  • Published:2013-02-15
  • Corresponding author: Cheng-jin HU
引用本文:

曹源, 王延群, 孙子涵, 孙黎, 韩凝, 杜秀敏, 孔立新, 綦瑞霞, 胡成进. 采用决策树方法建立基于UF-1000i尿液分析仪镜检规则的研究[J/OL]. 中华肾病研究电子杂志, 2013, 02(01): 38-41.

Yuan CAO, Yan-qun WANG, Zi-han SUN, Li SUN, Ning HAN, Xiu-min DU, Li-xin KONG, Ruixia QI, Cheng-jin HU. Construction of microscopy review rules based on UF-1000i using decision tree approach[J/OL]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2013, 02(01): 38-41.

目的

采用决策树的方法构建尿液镜检规则,以提高标本筛选的准确率。

方法

使用UF-1000i、Urisys 2400尿液干化学分析仪(以下简称Urisys 2400)及DiaSys RS 2003尿沉渣工作站(以下简称RS 2003)分析2000例尿液样本。根据其检查结果,定义“阳性样本”和“阴性样本”。在此基础上,构建训练集及测试集。并采用决策树的方法进行建模预测,以获得最优的镜检规则。

结果

基于决策树方法构建的尿液镜检规则,镜检率能控制在25.0%左右,敏感度为85.0%,特异度为96.4%。与已有镜检规则相比,该镜检规则的镜检率较低。

结论

决策树方法可以用于尿液分析镜检规则的建立,该方法对现有的镜检规则是有益的补充。

Objective

To get better accuracy, microscopic review rules of urine sediments were built based on a decision tree approach.

Methods

A total of 2000 urine samples were examined using UF-1000i, Urisys-2400, and RS 2003 urine sediment workstation, respectively. Positive and negative samples were defined, and both the training and testing sets were set up. Finally, a decision tree approach was employed to construct classifiers for screening urine samples.

Results

Using the decision tree method, we obtained a sensitivity of 85.0%, a specificity of 96.4% and a total review rate of 25.0% on the testing set, showing the acceptable sensitivity and lower total review rate comparing with the existing method.

Conclusion

An algorithm based on the decision tree for building review criteria can be available, which is valuable and a supplement for other microscopic review rules.

表1 尿流式细胞仪UF-1000i与尿沉渣工作站RS 2003两种方法检测2000例受试者尿液样本的批内重复性检测结果
图1 2000例受试者尿液样本采用决策树方法和专家系统方法预测结果的ROC曲线 注:黑色为决策树方法,蓝色为专家系统方法
表2 2000例受试者尿液样本量及年龄和性别比例与训练集和测试集的检测结果分布
表3 决策树与专家系统两种方法检测2000例受试者尿液样本的镜检规则比较
图2 基于决策树方法构建的镜检规则 注:Cond.为电导率,P.CAST为病理管型,CAST为管型,SRC为小圆上皮细胞,S_SSC为侧向散射光强度,RBC为红细胞计数,BLD为潜血结果,CRYSTA为结晶,RBC-P70Fsc为70%红细胞的前向散射光强度,Node Positive sample(阳性结果)为应该进行显微镜镜检复查的样本,Node Negative sample(阴性结果)可以不用复查直接发放报告的样本
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