特发性膜性肾病患者尿外泌体miRNA-193a表达的研究

doi:10.3877/cma.j.issn.2095-3216.2017.02.005

中华肾病研究电子杂志 ›› 2017, Vol. 06 ›› Issue (02) : 69 -72. doi: 10.3877/cma.j.issn.2095-3216.2017.02.005

所属专题：文献；

论著

特发性膜性肾病患者尿外泌体miRNA-193a表达的研究

刘书馨¹, 常明¹^,^†(

), 刘杨¹, 梁雪², 康喆¹

^1. 116033　辽宁，大连市中心医院肾内科
^2. 116021　大连市第五人民医院肾内科

收稿日期:2016-12-30 出版日期:2017-04-28
通信作者: 常明
基金资助:
辽宁省自然科学基金(2013020224); 大连市科技局计划项目(2014E14SF151)

Urine exosome expression of miRNA-193a in patients with idiopathic membranous nephropathy

Shuxin Liu¹, Ming Chang¹^,^†(), Yang Liu¹, Xue Liang², Zhe Kang¹

^1. Department of Nephrology, Dalian Municipal Central Hospital
^2. Department of Nephrology, Dalian Municipal Fifth Hospital; Dalian 116033, China

Received:2016-12-30 Published:2017-04-28
Corresponding author: Ming Chang
About author:
Corresponding author: Chang Ming, Email: mingchang@yeah.net

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目的

探讨特发性膜性肾病(IMN)患者尿外泌体miRNA-193a的表达变化。

方法

选取年龄、性别匹配的IMN肾病综合征患者(IMN肾病综合征组)、IMN缓解患者(IMN缓解组)各14例及正常对照组14名。利用Exoquick试剂盒提取三组尿样本外泌体，并提取尿外泌体中RNA，利用实时荧光定量逆转录聚合酶链反应(qRT-PCR)对miRNA-193a表达量进行测定。采用SPSS19.0软件进行统计学分析。

结果

三组间尿外泌体miRNA-193a的表达存在差异，以正常对照组的2-△△CT为1作为基准校正，IMN患者尿外泌体miRNA-193a的表达明显高于正常对照组(相对表达倍数为4.30±0.66)，差异有统计学意义(t＝4.579，P<0.05)；其中IMN肾病综合征组尿外泌体miRNA-193a表达显著高于IMN缓解组(相对表达倍数6.07±0.72与2.54±0.43)，差异有统计学意义(t＝3.282，P<0.05)。

结论

IMN患者尿外泌体miRNA -193a表达显著高于正常组，并且随着疾病缓解表达下调。该结果提示尿外泌体miRNA-193a在IMN的发生发展过程中可能起到一定的作用。

关键词: 特发性膜性肾病, 外泌体, 微小RNA

Objective

To explore the miRNA-193a expression in urine exosomes of idiopathic membranous nephropathy (IMN) patients, and to lay the foundation for studying the role of urinary exosomes miRNA in development of IMN.

Methods

Age and gender-matched IMN patients and normal control were selected and divided into IMN nephrotic syndrome group, IMN remission group, and normal control group with 14 cases each. Urine exosomes of IMN nehprotic patients, IMN complete or partial remission patients, and heathy controls were isolated by ExoQuick Exosome precipitaion solution. Exosomal RNA was extracted by Trizol kits. The expression of miRNA-193a and miRNA-168a were measured by real-time qRT-PCR.

Results

The urine exosome miRNA-193a expression of IMN patients was much higher than that of healthy controls (relative expression ratio 4.30 ± 0.66 vs 1.00, P<0.05). Meanwhile, the urine exosomal miRNA-193a expression of IMN nehprotic patients was significantly higher than that of IMN complete or partial remission patients (relative expression ratio 6.07±0.72 vs 2.54±0.43, P<0.05).

Conclusions

The urine exosome miRNA-193a expression of IMN patients was much higher than that of healthy controls, and downregulated as remission was achieved. The results suggested that miRNA-193a might play a role in the pathogenesis of IMN.

Key words: Idiopathic membranous nephropathy, Exosome, MicroRNA

图1 内参miR-168a扩增曲线

图2 尿外泌体miRNA-193a部分扩增曲线

图3 三组尿外泌体miRNA-193a的相对表达

表1 特发性膜性肾病患者及正常对照组的临床指标(±s)

组别	例数	年龄(岁)	尿蛋白定量(mg/24 h)	血清白蛋白(g/L)	肌酐(μmol/L)	尿素氮(mmol/L)
IMN肾病综合征组	14	48.29±14.22	4145.2±1018.3^b	23.81±5.25^ab	66.71±14.58	6.00±2.41^a
IMN缓解组	14	45.71±14.32	511.6±382.4	38.86±4.91^a	62.21±10.72	5.50±1.67
正常对照组	14	49.93±14.15	－	45.94±6.20	59.71±12.23	4.31±1.06
组别	例数	总胆固醇(mmol/L)	三酰甘油(mmol/L)	高密度脂蛋白胆固醇(mmol/L)	低密度脂蛋白胆固醇(mmol/L)	血红蛋白(g/L)
IMN肾病综合征组	14	9.36±3.71^ab	1.96±1.14^a	1.62±0.70^b	4.91±2.27^ab	128.71±18.84
IMN缓解组	14	4.94±0.95	1.68±0.81	1.35±0.35	3.18±1.74	129.36±14.44
正常对照组	14	4.08±1.05	1.26±0.45	1.91±0.88	2.64±0.66	135.50±7.80

表1 特发性膜性肾病患者及正常对照组的临床指标(±s)

组别	例数	年龄(岁)	尿蛋白定量(mg/24 h)	血清白蛋白(g/L)	肌酐(μmol/L)	尿素氮(mmol/L)
IMN肾病综合征组	14	48.29±14.22	4145.2±1018.3^b	23.81±5.25^ab	66.71±14.58	6.00±2.41^a
IMN缓解组	14	45.71±14.32	511.6±382.4	38.86±4.91^a	62.21±10.72	5.50±1.67
正常对照组	14	49.93±14.15	－	45.94±6.20	59.71±12.23	4.31±1.06
组别	例数	总胆固醇(mmol/L)	三酰甘油(mmol/L)	高密度脂蛋白胆固醇(mmol/L)	低密度脂蛋白胆固醇(mmol/L)	血红蛋白(g/L)
IMN肾病综合征组	14	9.36±3.71^ab	1.96±1.14^a	1.62±0.70^b	4.91±2.27^ab	128.71±18.84
IMN缓解组	14	4.94±0.95	1.68±0.81	1.35±0.35	3.18±1.74	129.36±14.44
正常对照组	14	4.08±1.05	1.26±0.45	1.91±0.88	2.64±0.66	135.50±7.80

[1]	Li LS, Liu ZH. Epidemiologic data of renal diseases from a single unit in China: analysis based on 13，519 renal biopsies [J]. Kidney Int, 2004, 66(3): 920-923.
[2]	Debiec H, Guigonisv V, Mougenot B, et al. Antenatal membranous glomerulonephritis due to anti-neutral endopeptidase antibodies [J]. N Engl J Med, 2002, 346(26): 2053-2060.
[3]	Hobert O. Gene regulation by transcription factors and microRNAs [J]. Science, 2008, 319(5871): 1785-1786.
[4]	Chung AC, Yu X, Lan HY. MicroRNA and nephropathy: emerging concepts [J]. Int J Nephrol Renovasc Dis, 2013, 6: 169-179.
[5]	Wang G, Tam LS, Kwan BC, et al. Expression of miR-146a and miR-155 in the urinary sediment of systemic lupus erythematosus [J]. Clin Rheumatol, 2012, 31(3): 435-440.
[6]	Wang G, Kwan BC, Lai FM, et al. Urinary sediment miRNA levels in adult nephrotic syndrome [J]. Clin Chim Acta, 2013, 418: 5-11.
[7]	Zhang C, Zhang W, Chen HM, et al. Plasma microRNA-186 and proteinuria in focal segmental glomerulosclerosis [J]. Am J Kidney Dis, 2015, 65(2): 223-232.
[8]	Gebeshuber CA, Kornauth C, Dong L, et al. Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1 [J]. Nat Med, 2013, 19(4): 481-487.
[9]	Gonzales PA, Pisitkun T, Hoffert JD, et al. Large-scale proteomics and phosphoproteomics of urinary exosomes [J]. J Am Soc Nephrol, 2009, 20(2): 363- 379.
[10]	Miranda KC, Bond DT, McKee M, et al. Nucleic acids within urinary exosome/microvesicles are potential biomakers for renal disease [J]. Kidney Int, 2010, 78(2): 191- 199.
[11]	Van Balkom BW, Pisitkun T, Verhaar MC, et al. Exosomes and the kidney: prospects for diagnosis and therapy of renal diseases [J]. Kidney Int, 2011, 80(11): 1138- 1145.
[12]	Chen SY, Chen CH, Huang YC, et al. Genetic susceptibility to idiopathic membranous nephropathy in high-prevalence area, Taiwan [J]. Biomedicine (Taipei), 2014, 4: 9.
[13]	Kavoura E, Gakiopoulou H, Paraskevakou H, et al. Immunohistochemical evaluation of podocalyxin expression in glomerulopathies associated with nephrotic syndrome [J]. Hum Pathol, 2011, 42(2): 227-235.
[14]	Huang Z, Zhang Y, Zhou J, et al. Urinary exosomal miR-193a can be a potential biomarker for the diagnosis of primary focal segmental glomerulosclerosis in children [J]. Biomed Res Int, 2017, 2017: 7298160.

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