急性肾损伤-慢性肾脏病转化小鼠模型制备的教学要点及学习效果分析

doi:10.3877/cma.j.issn.2095-3216.2024.04.008

中华肾病研究电子杂志 ›› 2024, Vol. 13 ›› Issue (04) : 226 -230. doi: 10.3877/cma.j.issn.2095-3216.2024.04.008

教学园地

急性肾损伤-慢性肾脏病转化小鼠模型制备的教学要点及学习效果分析

袁楠¹, 黄梦杰², 白云凤², 李晓帆², 罗从娟³^,^†(

), 陈健文²^,^†(

)

^1. 100853　北京，解放军总医院第一医学中心肾脏病医学部、肾脏疾病全国重点实验室、国家慢性肾病临床医学研究中心、肾脏疾病研究北京市重点实验室；266003　青岛大学附属医院肾病科
^2. 100853　北京，解放军总医院第一医学中心肾脏病医学部、肾脏疾病全国重点实验室、国家慢性肾病临床医学研究中心、肾脏疾病研究北京市重点实验室
^3. 266003　青岛大学附属医院肾病科

收稿日期:2024-06-05 出版日期:2024-08-28
通信作者: 罗从娟, 陈健文
基金资助:
国自然科学基金青年项目(82100713); 中国博士后科学基金特别资助(2021T140791)

Analysis of teaching points and learning effect in the establishment of an acute kidney injury-chronic kidney disease transition model in mice

Nan Yuan¹, Mengjie Huang², Yunfeng Bai², Xiaofan Li², Congjuan Luo³^,^†(), Jianwen Chen²^,^†()

^1. Department of Nephrology, First Medical Center of Chinese PLA General Hospital, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853; Department of Nephrology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province; China
^2. Department of Nephrology, First Medical Center of Chinese PLA General Hospital, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853
^3. Department of Nephrology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province; China

Received:2024-06-05 Published:2024-08-28
Corresponding author: Congjuan Luo, Jianwen Chen

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引用本文：

袁楠, 黄梦杰, 白云凤, 李晓帆, 罗从娟, 陈健文. 急性肾损伤-慢性肾脏病转化小鼠模型制备的教学要点及学习效果分析[J]. 中华肾病研究电子杂志, 2024, 13(04): 226-230.

Nan Yuan, Mengjie Huang, Yunfeng Bai, Xiaofan Li, Congjuan Luo, Jianwen Chen. Analysis of teaching points and learning effect in the establishment of an acute kidney injury-chronic kidney disease transition model in mice[J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2024, 13(04): 226-230.

目的

分析小鼠急性肾损伤-慢性肾脏病(AKI-CKD)转化模型制备教学要点及学习效果。

方法

教学对象为未做过动物实验的研究生4例，由资深教师进行14 d的制备小鼠AKI-CKD转化模型的培训。以小鼠术前死亡率、右侧肾脏肾蒂结扎成功率、下腔静脉穿刺取血成功率、建模所需时间等指标对教学效果进行评估分析。

结果

与第1天相比，培训第7天时的小鼠术前死亡率(3.3%)显著下降，而且右侧肾脏结扎成功率(97.06%)及下腔静脉取血成功率(98.44%)也明显提高。经过14 d培训，建模所需时间从第1天的平均248 s缩短到平均58 s。

结论

以术前死亡率、右侧肾脏结扎成功率、下腔静脉取血成功率和建模所需时间为教学要点的14 d培训，可以使参训研究生掌握小鼠AKI-CKD转化模型的制备方法。

关键词: 医学研究生教学, 急性肾损伤慢性化转变, 动物模型, 学习效果

Objective

To analyze the teaching points and learning effect of an acute kidney injury-chronic kidney disease (AKI-CKD) transition model in mice.

Methods

The teaching objects were 4 graduate students who had not done animal experiments. Senior teachers conducted the 14-day training to prepare the mouse AKI-CKD transition model. The teaching effect was evaluated by the indexes including the preoperative mortality, the success rate of right kidney pedicle ligation, the success rate of blood obtaining by the inferior vena cava puncture, and the time of model preparation.

Results

Compared with results of the first day, results of seventh day displayed that the preoperative mortality (3.3%) was significantly decreased, and both the success rate of right kidney pedicle ligation (97.06%) and the success rate of blood obtaining by the inferior vena cava puncture (98.44%) were also significantly increased. After the 14-day training, the time of mouse model preparation was reduced to an average of 58 seconds from an average of 248 seconds on the first day.

Conclusion

The 14-day training with the preoperative mortality rate, the success rate of right kidney pedicle ligation, the success rate of blood extraction by inferior vena cava puncture, and the time of mouse model preparation as the teaching points could enable the participating graduate students to master the preparation method of mouse AKI-CKD transition model.

Key words: Medical graduate teaching, Chronic transition of acute kidney injury, Animal model, Learning effect

图1 小鼠术前死亡率注：与培训第1天比较，^aP<0.001；与培训第3天比较，^bP<0.001

图2 右侧肾脏结扎成功率注：与培训第1天比较，^aP<0.001；与培训第3天比较，^bP<0.001

图3 下腔静脉取血成功率注：与培训第1天比较，^aP<0.001；与培训第3天比较，^bP<0.001

图4 造模所需时间测评注：与培训第1天比较，^aP<0.001；与培训第3天比较，^bP<0.001

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