Expression of thrombospondin-1 and its receptor CD47 in vascular lesions of renal interstitial fibrosis

doi:10.3877/cma.j.issn.2095-3216.2015.01.007

Abstract

Abstract:

Objective

To observe the expression of TSP-1 and its receptor CD47 in unilateral ureteral obstruction (UUO) rats, and to investigate the role of TSP-1-CD47 in lesions of peritubular capillary (PTC).

Methods

A total of 60 Sprague-Dawley rats were randomly divided into: sham-operation group (SOR) (n=15) and UUO group (n=45). UUO group sacrifice timing consisted of three time points: 3 day, 7 day, 14 day, with 15 animals for each time point. Detection of 24h urinary protein and renal function was made by automatic biochemistry analyzer, and interstitial pathological changes were shown by Masson staining. The expression of TSP-1 and CD47 was measured by immunohistochemical and immunofluorescence double staining. Western blot was used to detect the expression of CD34, VEGF, TSP-1 protein in rat kidneys. The correlations among CD34, VEGF, and TSP-1 protein expression were analyzed with Pearson method.

Results

Compared with the sham-operation group, the levels of serum creatinine (Scr), blood urea nitrogen (BUN), and 24h urinary protein did not change significantly in UUO group, but features of renal tubulointerstitial fibrosis were obvious, and the score of renal interstitial lesion was significantly higher in UUO group. Immunofluorescence double staining showed that TSP-1 and CD47 were coexpressed in UUO renal tubules. Western blot confirmed that TSP-1 protein level was significantly higher in UUO group, while the expressions of CD34 and VEGF protein were decreased. Correlation analysis showed that TSP-1 protein was negatively associated with the expression of CD34 and VEGF(r=-0.931, P<0.01; r=-0.953, P<0.01).

Conclusion

TSP-1 and its receptor CD47 were coexpressed in UUO rats, and negatively associated with PTC. TSP-1 may be involved in the pathological changes of PTC through CD47 in UUO kidneys.

Key words: Unilateral ureteral obstruction, Thrombospondin-1, CD47, Peritubular capillary lesion

Mengdi Xia, Xisheng Xie, Yanni Zhang. Expression of thrombospondin-1 and its receptor CD47 in vascular lesions of renal interstitial fibrosis[J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2015, 04(01): 37-42.

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References 18

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组别	只数	尿蛋白(mg/24 h)	血清肌酐(μmol/L)	尿素氮(mmol/L)
假手术3 d组	4	18.18±3.78	39.86±2.80	5.94±0.77
假手术7 d组	5	21.12±2.85	37.30±2.24	5.86±1.24
假手术14 d组	4	18.50±3.49	40.18±1.95	6.05±1.15
UUO 3 d组	15	19.70±3.93	45.04±6.77	5.96±0.92
UUO 7 d组	12	20.92±5.41	45.76±4.22	5.76±1.05
UUO 14 d组	11	20.89±3.96	44.75±5.51	5.89±1.03
F值	?	0.572	0.568	3.904
P值	?	0.885	0.888	0.096

组别	只数	尿蛋白(mg/24 h)	血清肌酐(μmol/L)	尿素氮(mmol/L)
假手术3 d组	4	18.18±3.78	39.86±2.80	5.94±0.77
假手术7 d组	5	21.12±2.85	37.30±2.24	5.86±1.24
假手术14 d组	4	18.50±3.49	40.18±1.95	6.05±1.15
UUO 3 d组	15	19.70±3.93	45.04±6.77	5.96±0.92
UUO 7 d组	12	20.92±5.41	45.76±4.22	5.76±1.05
UUO 14 d组	11	20.89±3.96	44.75±5.51	5.89±1.03
F值	?	0.572	0.568	3.904
P值	?	0.885	0.888	0.096

组别	CD34/β-actin	VEGF/β-actin	TSP-1/β-actin
假手术组	8243±534.6	2878.2±294.9	799.2±57.5
UUO7 d组	5443.4±546.4^a	1915.1±157.0^a	1492.5.86±162.9^a
UUO14d组	2753.6±650.8^a	851.8±82.3^a	2367.2±182.1^a

组别	CD34/β-actin	VEGF/β-actin	TSP-1/β-actin
假手术组	8243±534.6	2878.2±294.9	799.2±57.5
UUO7 d组	5443.4±546.4^a	1915.1±157.0^a	1492.5.86±162.9^a
UUO14d组	2753.6±650.8^a	851.8±82.3^a	2367.2±182.1^a

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