Urine exosome expression of miRNA-193a in patients with idiopathic membranous nephropathy

doi:10.3877/cma.j.issn.2095-3216.2017.02.005

Abstract

Abstract:

Objective

To explore the miRNA-193a expression in urine exosomes of idiopathic membranous nephropathy (IMN) patients, and to lay the foundation for studying the role of urinary exosomes miRNA in development of IMN.

Methods

Age and gender-matched IMN patients and normal control were selected and divided into IMN nephrotic syndrome group, IMN remission group, and normal control group with 14 cases each. Urine exosomes of IMN nehprotic patients, IMN complete or partial remission patients, and heathy controls were isolated by ExoQuick Exosome precipitaion solution. Exosomal RNA was extracted by Trizol kits. The expression of miRNA-193a and miRNA-168a were measured by real-time qRT-PCR.

Results

The urine exosome miRNA-193a expression of IMN patients was much higher than that of healthy controls (relative expression ratio 4.30 ± 0.66 vs 1.00, P<0.05). Meanwhile, the urine exosomal miRNA-193a expression of IMN nehprotic patients was significantly higher than that of IMN complete or partial remission patients (relative expression ratio 6.07±0.72 vs 2.54±0.43, P<0.05).

Conclusions

The urine exosome miRNA-193a expression of IMN patients was much higher than that of healthy controls, and downregulated as remission was achieved. The results suggested that miRNA-193a might play a role in the pathogenesis of IMN.

Key words: Idiopathic membranous nephropathy, Exosome, MicroRNA

Shuxin Liu, Ming Chang, Yang Liu, Xue Liang, Zhe Kang. Urine exosome expression of miRNA-193a in patients with idiopathic membranous nephropathy[J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2017, 06(02): 69-72.

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URL: https://zhsbyjdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-3216.2017.02.005

https://zhsbyjdzzz.cma-cmc.com.cn/EN/Y2017/V06/I02/69

Figures/Tables 4

References 14

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组别	例数	年龄(岁)	尿蛋白定量(mg/24 h)	血清白蛋白(g/L)	肌酐(μmol/L)	尿素氮(mmol/L)
IMN肾病综合征组	14	48.29±14.22	4145.2±1018.3^b	23.81±5.25^ab	66.71±14.58	6.00±2.41^a
IMN缓解组	14	45.71±14.32	511.6±382.4	38.86±4.91^a	62.21±10.72	5.50±1.67
正常对照组	14	49.93±14.15	－	45.94±6.20	59.71±12.23	4.31±1.06
组别	例数	总胆固醇(mmol/L)	三酰甘油(mmol/L)	高密度脂蛋白胆固醇(mmol/L)	低密度脂蛋白胆固醇(mmol/L)	血红蛋白(g/L)
IMN肾病综合征组	14	9.36±3.71^ab	1.96±1.14^a	1.62±0.70^b	4.91±2.27^ab	128.71±18.84
IMN缓解组	14	4.94±0.95	1.68±0.81	1.35±0.35	3.18±1.74	129.36±14.44
正常对照组	14	4.08±1.05	1.26±0.45	1.91±0.88	2.64±0.66	135.50±7.80

组别	例数	年龄(岁)	尿蛋白定量(mg/24 h)	血清白蛋白(g/L)	肌酐(μmol/L)	尿素氮(mmol/L)
IMN肾病综合征组	14	48.29±14.22	4145.2±1018.3^b	23.81±5.25^ab	66.71±14.58	6.00±2.41^a
IMN缓解组	14	45.71±14.32	511.6±382.4	38.86±4.91^a	62.21±10.72	5.50±1.67
正常对照组	14	49.93±14.15	－	45.94±6.20	59.71±12.23	4.31±1.06
组别	例数	总胆固醇(mmol/L)	三酰甘油(mmol/L)	高密度脂蛋白胆固醇(mmol/L)	低密度脂蛋白胆固醇(mmol/L)	血红蛋白(g/L)
IMN肾病综合征组	14	9.36±3.71^ab	1.96±1.14^a	1.62±0.70^b	4.91±2.27^ab	128.71±18.84
IMN缓解组	14	4.94±0.95	1.68±0.81	1.35±0.35	3.18±1.74	129.36±14.44
正常对照组	14	4.08±1.05	1.26±0.45	1.91±0.88	2.64±0.66	135.50±7.80

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