Exploration of the method to transplant mesenchymal stem cells under the capsule of mouse kidney

doi:10.3877/cma.j.issn.2095-3216.2021.06.005

Chinese Journal of Kidney Disease Investigation(Electronic Edition) ›› 2021, Vol. 10 ›› Issue (06): 323-327. doi: 10.3877/cma.j.issn.2095-3216.2021.06.005

• Original Article • Previous Articles Next Articles

Exploration of the method to transplant mesenchymal stem cells under the capsule of mouse kidney

Duo Li¹, Mengjie Huang², Shang Chen³, Zongjin Li³, Jianwen Chen²^,^†(), Xiangmei Chen⁴^,^†()

^1. Department of Nephrology, First Medical Center of Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853; Tianjin University Institute of Disaster and Emergency Medicine, Tianjin 300071; Department of Oncology, First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453003, Henan Province
^2. Department of Nephrology, First Medical Center of Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853
^3. Nankai University School of Medicine, Tianjin 300071; China
^4. Department of Nephrology, First Medical Center of Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853; Nankai University School of Medicine, Tianjin 300071; China

Received:2021-08-16 Online:2021-12-28 Published:2022-01-05
Contact: Jianwen Chen, Xiangmei Chen

Abstract

Abstract:

Objective

To explore the transplantation of mesenchymal stem cells (MSCs) under the kidney capsule for the purpose of increasing the effective transplantation volume and retention rate of cells.

Methods

Firstly, parenchymal injection and subcapsular injection of mouse kidneys were performed with ink respectively to preliminarily explore the effect and feasibility. Then the MSCs were co-cultured with collagen matrix to investigate the conditions of cell survival and growth. The collagen/MSCs transplantation mice were divided into renal parenchymal transplantation group and renal subcapsular transplantation group. After transplantation, the kidneys were weighed and observed with HE staining. Mice of the renal subcapsular transplantation group were again divided into single collagen transplantation group and collagen plus MSCs transplantation group. In vivo imaging observations were performed at different time points after the operation.

Results

Compared with renal parenchymal injection, renal subcapsular injection made the ink cover a larger range of renal tissue and had a bigger bearing capacity. The MSCs grew well in the collagen matrix. After the renal subcapsular injection of collagen and MSCs suspension, the MSCs showed an effective and stable transplantation amount, and remained for a longer time.

Conclusion

The renal subcapsular transplantation showed that the amount of collagen-wrapped MSCs was stable and retained for a longer time with a certain feasibility. This study provided a new idea for MSCs transplantation in treating kidney injury, being of potential value for clinical application.

Key words: Mice, Stem cell transplantation, Renal capsule, Retention rate

Duo Li, Mengjie Huang, Shang Chen, Zongjin Li, Jianwen Chen, Xiangmei Chen. Exploration of the method to transplant mesenchymal stem cells under the capsule of mouse kidney[J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2021, 10(06): 323-327.

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Figures/Tables 4

References 12

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