Protective effect of omentum-encapsulated gelatin-mesenchymal stem cells complex on aristolochic acid nephropathy

doi:10.3877/cma.j.issn.2095-3216.2022.01.002

Abstract

Abstract:

Objective

To investigate the repair and protective effects of omentum-encapsulated gelatin-mesenchymal stem cells (MSCs) complex on the kidneys of mice with aristolochic acid nephropathy (AAN).

Methods

The MSCs-gelatin complex was made by co-culture of gelatin and MSCs. The biocompatibility between MSCs and gelatin as well as the proliferation ability of MSCs were evaluated with the CCK-8. Twenty-four healthy male C57BL/6 mice weighing 20-25 g were randomly divided into 4 groups: control group (normal mice), AAN model group (intraperitoneal injection of aristolochic acid solution), MSCs group (greater omentum-encapsulated MSCs), and MSCs-Gel group (greater omentum-encapsulated MSCs plus gelatin complex). An in vivo fluorescence imaging system was used to observe the distribution and survival of MSCs in mice. The levels of serum creatinine and blood urea nitrogen of the mice were measured. The renal histological changes and fibrosis indicators such as α-SMA, TGF-β, and collagen were also evaluated.

Results

The gelatin had no significant influence on the proliferation ability of MSCs. Compared with the MSCs group, the MSC-Gel group showed significantly longer survival of the MSCs in the mice kidneys, lower levels of serum creatinine and blood urea nitrogen, less expression of α-SMA, TGF-β, and collagen Ⅰ, and lower level of renal fibrosis (P<0.05).

Conclusion

The omentum-encapsulated gelatin-MSCs complex had good repair and protective effects on the kidneys of AAN mice.

Key words: Greater omentum, Gelatin, Mesenchymal stem cells, Aristolochic acid nephropathy

Zhangning Fu, Chengcheng Song, Xiaodong Geng, Kun Chi, Quan Hong, Di Wu. Protective effect of omentum-encapsulated gelatin-mesenchymal stem cells complex on aristolochic acid nephropathy[J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2022, 11(01): 7-14.

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References 40

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