Application of immunoelectron microscopy in diagnosis and research of renal diseases

doi:10.3877/cma.j.issn.2095-3216.2022.04.007

Abstract

Abstract:

Immunoelectron microscopy is a combination of antigen-antibody reaction and electron microscopy, which can be used to observe and analyze antigens at subcellular and ultrastructural level. Its applications in diagnosis and research of renal diseases involve monoclonal immunoglobulin deposition nephropathy, immune complex-mediated glomerular diseases, glomerular structure research, pathogenesis of renal tubular diseases, and other renal diseases. This review comprehensively showed the important role and development trend of immunoelectron microscopy in diagnosis and research of renal diseases, in order to support the future research in related fields.

Key words: Immunoelectron microscopy, Kidney diseases, Glomerulus, Renal tubule

Wanjun Shen, Tiantian Wang, Zhiwei Yin, Yuansheng Xie. Application of immunoelectron microscopy in diagnosis and research of renal diseases[J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2022, 11(04): 219-223.

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References 45

[1]	Singer SJ. Preparation of an electron-dense antibody conjugate [J]. Nature, 1959, 183(4674): 1523-1524.
[2]	Roth J, Bendayan M, Orci L. Ultrastructural localization of intracellular antigens by the use of protein A-gold complex [J]. J Histochem Cytochem, 1978, 26(12): 1074-1081.
[3]	Kim HL, Riew TR, Park J, et al. Correlative light and electron microscopy using frozen section obtained using cryo-ultramicrotomy [J]. Int J Mol Sci, 2021, 22(8): 4273.
[4]	Najafian B, Lusco MA, Alpers CE, et al. Approach to kidney biopsy: core curriculum 2022 [J]. Am J Kidney Dis, 2022, 80(1): 119-131.
[5]	Gertz MA. Immunoglobulin light chain amyloidosis: 2022 update on diagnosis, prognosis, and treatment [J]. Am J Hematol, 2022, 97(6): 818-829.
[6]	Sethi S, Rajkumar SV, D′agati VD. The complexity and heterogeneity of monoclonal immunoglobulin-associated renal diseases [J]. J Am Soc Nephrol, 2018, 29(7): 1810-1823.
[7]	Said SM, Best Rocha A, Valeri AM, et al. The characteristics of patients with kidney light chain deposition disease concurrent with light chain amyloidosis [J]. Kidney Int, 2022, 101(1): 152-163.
[8]	Kanzaki G, Okabayashi Y, Nagahama K, et al. Monoclonal immunoglobulin deposition disease and related diseases [J]. J Nippon Med Sch, 2019, 86(1): 2-9.
[9]	Benson MD, Buxbaum JN, Eisenberg DS, et al. Amyloid nomenclature 2018: recommendations by the International Society of Amyloidosis (ISA) nomenclature committee [J]. Amyloid, 2018, 25(4): 215-219.
[10]	陈思羽，韩伟霞，王晨. 肾淀粉样变性病的病理诊断与分型、分级的研究进展[J/CD]. 中华肾病研究电子杂志，2020, 9 (2): 86-89.
[11]	Satoskar AA, Burdge K, Cowden DJ, et al. Typing of amyloidosis in renal biopsies: diagnostic pitfalls [J]. Arch Pathol Lab Med, 2007, 131(6): 917-922.
[12]	Lachmann HJ, Booth DR, Booth SE, et al. Misdiagnosis of hereditary amyloidosis as AL (primary) amyloidosis [J]. N Engl J Med, 2002, 346(23): 1786-1791.
[13]	Abildgaard N, Rojek AM, Moller HE, et al. Immunoelectron microscopy and mass spectrometry for classification of amyloid deposits [J]. Amyloid, 2020, 27(1): 59-66.
[14]	Heilman RL, Velosa JA, Holley KE, et al. Long-term follow-up and response to chemotherapy in patients with light-chain deposition disease [J]. Am J Kidney Dis, 1992, 20(1): 34-41.
[15]	Wang Q, Jiang F, Xu G. The pathogenesis of renal injury and treatment in light chain deposition disease [J]. J Transl Med, 2019, 17(1): 387.
[16]	王梅，王英，王素霞，等. 早期轻链沉积病的诊断 [J]. 中华肾脏病杂志，2004, 20(2): 87-89.
[17]	王素霞，邹万忠，王梅，等. 肾轻链沉积病和轻链型淀粉样变的电镜及免疫电镜研究 [J]. 北京大学学报(医学版), 2003, 35(6): 576-580.
[18]	Fogo AB, Lusco MA, Najafian B, et al. AJKD atlas of renal pathology: light chain proximal tubulopathy [J]. Am J Kidney Dis, 2016, 67(2): e9-e10.
[19]	Büttner-Herold M, Krieglstein N, Chuva T, et al. Light chain restriction in proximal tubules-implications for light chain proximal tubulopathy [J]. Front Med (Lausanne), 2022, 9: 723758.
[20]	Li XM, Xu F, Liang DD, et al. Clinicopathologic characteristics of light chain proximal tubulopathy with light chain inclusions involving multiple renal cell types [J]. Clin Nephrol, 2018, 89(2): 83-92.
[21]	Kapur U, Barton K, Fresco R, et al. Expanding the pathologic spectrum of immunoglobulin light chain proximal tubulopathy [J]. Arch Pathol Lab Med, 2007, 131(9): 1368-1372.
[22]	Gu X, Barrios R, Cartwright J, et al. Light chain crystal deposition as a manifestation of plasma cell dyscrasias: the role of immunoelectron microscopy [J]. Hum Pathol, 2003, 34(3): 270-277.
[23]	Hoxha E, Reinhard L, Stahl RAK. Membranous nephropathy: new pathogenic mechanisms and their clinical implications [J]. Nat Rev Nephrol, 2022, 18(7): 466-478.
[24]	Prunotto M, Carnevali ML, Candiano G, et al. Autoimmunity in membranous nephropathy targets aldose reductase and SOD2 [J]. J Am Soc Nephrol, 2010, 21(3): 507-519.
[25]	Gödel M, Grahammer F, Huber TB. Thrombospondin type-1 domain-containing 7A in idiopathic membranous nephropathy [J]. N Engl J Med, 2015, 372(11): 1073-10755.
[26]	Herwig J, Skuza S, Sachs W, et al. Thrombospondin type 1 domain-containing 7A localizes to the slit diaphragm and stabilizes membrane dynamics of fully differentiated podocytes [J]. J Am Soc Nephrol, 2019, 30(5): 824-839.
[27]	Rodrigues JC, Haas M, Reich HN. IgA nephropathy [J]. Clin J Am Soc Nephrol, 2017, 12(4): 677-686.
[28]	Lai KN, Tang SC, Schena FP, et al. IgA nephropathy [J]. Nat Rev Dis Primers, 2016, 2: 16001.
[29]	Nishioka R, Hara S, Kawahara H, et al. Glomerulonephritis with severe nephrotic syndrome induced by immune complexes composed of galactose-deficient IgA1 in primary Sjogren′s syndrome: a case report [J]. BMC Nephrol, 2021, 22(1): 108.
[30]	Dysart NK Jr, Sisson S, Vernier RL. Immunoelectron microscopy of IgA nephropathy [J]. Clin Immunol Immunopathol, 1983, 29(2): 254-270.
[31]	王素霞，邹万忠，杨莉，等. 膜性肾病合并IgA肾病的临床病理特点[J]. 中华病理学杂志，2007, 36(3): 171-174.
[32]	Sjowall C, Olin AI, Skogh T, et al. C-reactive protein, immunoglobulin G and complement co-localize in renal immune deposits of proliferative lupus nephritis [J]. Autoimmunity, 2013, 46(3): 205-214.
[33]	Horvei KD, Pedersen HL, Fismen S, et al. Lupus nephritis progression in FcγRIIB^－/－yaa mice is associated with early development of glomerular electron dense deposits and loss of renal DNase I in severe disease [J]. PLoS One, 2017, 12(11): e0188863.
[34]	Grahammer F, Wigge C, Schell C, et al. A flexible, multilayered protein scaffold maintains the slit in between glomerular podocytes [J]. JCI Insight, 2016, 1(9): e86177.
[35]	Yuan HP, Takeuchi E, Taylor GA, et al. Nephrin dissociates from actin, and its expression is reduced in early experimental membranous nephropathy [J]. J Am Soc Nephrol, 2002, 13(4): 946-956.
[36]	Roselli S, Gribouval O, Boute N, et al. Podocin localizes in the kidney to the slit diaphragm area [J]. Am J Pathol, 2002, 160(1): 131-139.
[37]	La TM, Tachibana H, Li SA, et al. Dynamin 1 is important for microtubule organization and stabilization in glomerular podocytes [J]. FASEB J, 2020, 34(12): 16449-16463.
[38]	Sachs M, Wetzel S, Reichel TJ, et al. ADAM10-mediated ectodomain shedding is an essential driver of podocyte damage [J]. J Am Soc Nephrol, 2021, 32(6): 1389-1408.
[39]	Nielsen R, Christensen EI, Birn H. Megalin and cubilin in proximal tubule protein reabsorption: from experimental models to human disease [J]. Kidney Int, 2016, 89(1): 58-67.
[40]	Sun J, Hultenby K, Axelsson J, et al. Proximal tubular expression patterns of megalin and cubilin in proteinuric nephropathies [J]. Kidney Int Rep, 2017, 2(4): 721-732.
[41]	胡小影. MC1R在肾脏中表达定位及AKI模型肾组织中的表达变化研究 [D]. 郑州：郑州大学，2021.
[42]	Cao Y, Zhang Y, Wang S, et al. Detection of the hepatitis C virus antigen in kidney tissue from infected patients with various glomerulonephritis [J]. Nephrol Dial Transplant, 2009, 24(9): 2745-2751.
[43]	Stamatiades EG, Tremblay ME, Bohm M, et al. Immune monitoring of trans-endothelial transport by kidney-resident macrophages [J]. Cell, 2016, 166(4): 991-1003.
[44]	李红梅，张勤奋，李茵茵，等. 透射电镜和光镜联合应用于细胞观察[J]. 实验室研究与探索，2019, 38(11): 18-20, 65.
[45]	Nasr SH, Fogo AB. New developments in the diagnosis of fibrillary glomerulonephritis [J]. Kidney Int, 2019, 96(3): 581-592.

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