Therapeutic effect of ultrasound microbubble-assisted intrarenal delivery of mesenchymal stem cellderived exosomes under ultrasound guidance on acute kidney injury in rats

doi:10.3877/cma.j.issn.2095-3216.2025.03.002

Abstract

Abstract:

Objective

This experiment aimed to explore the therapeutic effect of ultrasound microbubble-assisted intrarenal delivery of mesenchymal stem cell-derived exosomes under ultrasound guidance on acute kidney injury in rats.

Methods

Twenty-four male 8-week-old normal SD rats were randomly divided into four groups， with six rats in each group： the control group （normal rats injected with phosphate-buffered saline）， the model group （acute kidney injury rats injected with phosphate-buffered saline）， the exosome group （acute kidney injury rats injected with exosomes）， and the mixed vesicle group（acute kidney injury rats injected with ultrasound microbubble-mixed exosomes）.The rat model of acute kidney injury was established by intravenous injection of cisplatin （5 mg/kg） via the tail vein， while the control group rats received an equal volume of phosphate-buffered saline via the tail vein.On the first day after the establishment of the acute kidney injury model， percutaneous intrarenal injection was performed under ultrasound guidance in each group.A total of 100 μl liquid was injected into the upper and lower poles of the rat kidneys： the control group and the model group being injected with phosphate-buffered saline， while the exosome group was injected with exosomes，and the mixed vesicle group was injected with exosomes mixed with ultrasound microbubbles.Immediately after the above injections， ultrasound was applied with the same parameters for activation.The renal function of each group of rats was monitored daily after the injections，and tissue samples were collected on the 4th day.Kidney tissue pathology was observed， and the number of apoptotic cells as well as the expression of kidney injury molecule-1 （KIM-1） protein were detected.

Results

The ultrasound echogenic imaging effect of the mixed vesicle group was better than that of the exosome group.Moreover， the number of exosomes retained in the kidneys and the number of exosomes in the renal cells were both significantly increased in the mixed vesicle group compared to the exosome group （all P ＜0.05）.The renal function， renal tubular pathological injury score， number of apoptotic cells， and expression of KIM-1 protein in both the exosome group and the mixed vesicle group were better than those in the model group （all P ＜0.05）， and these parameters of the mixed vesicle group were also better than those of the exosome group（P ＜0.05）.

Conclusion

Ultrasound microbubble-assisted intrarenal delivery of mesenchymal stem cellderived exosomes under ultrasound guidance could alleviate acute kidney injury in the rats.

Key words: Acute kidney injury, Ultrasound microbubbles, Exosomes, Local delivery

Yuhao Chen, Chuyue Zhang, Chunjia Sheng, Tuo Xiao, Bo Jiang, Guangyan Cai. Therapeutic effect of ultrasound microbubble-assisted intrarenal delivery of mesenchymal stem cellderived exosomes under ultrasound guidance on acute kidney injury in rats[J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2025, 14(03): 126-132.

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Figures/Tables 7

References 13

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分组	基线水平	第1天	第2天	第3天	第4天
血清肌酐(μmol/L)
对照组	18.20±3.43	18.03±2.31^a	18.28±1.69^a	18.47±2.26^a	17.92±2.08^a
模型组	19.27±2.53	32.72±3.51	65.33±9.59	242.43±63.67	389.38±61.86
外泌体组	19.93±1.78	20.00±4.75^a	56.13±15.13	139.20±18.00^a	289.87±13.21^a
混合囊泡组	21.13±2.58	21.47±3.64^a	154.87±79.84^ab	131.78±54.93^a	175.82±28.58^ab
血尿素氮(mmol/L)
对照组	4.83±0.69	5.16±0.21^a	4.87±0.56^a	4.88±0.46^a	4.74±0.48^a
模型组	5.11±0.49	7.52±0.81	13.55±1.99	62.90±16.52	101.03±16.05
外泌体组	4.45±1.06	6.13±1.33	13.77±2.22^a	29.89±6.58^a	52.37±8.38^a
混合囊泡组	5.10±0.79	4.43±1.23^ab	34.75±16.84^a	27.76±11.93^a	35.03±11.18^ab

分组	基线水平	第1天	第2天	第3天	第4天
血清肌酐(μmol/L)
对照组	18.20±3.43	18.03±2.31^a	18.28±1.69^a	18.47±2.26^a	17.92±2.08^a
模型组	19.27±2.53	32.72±3.51	65.33±9.59	242.43±63.67	389.38±61.86
外泌体组	19.93±1.78	20.00±4.75^a	56.13±15.13	139.20±18.00^a	289.87±13.21^a
混合囊泡组	21.13±2.58	21.47±3.64^a	154.87±79.84^ab	131.78±54.93^a	175.82±28.58^ab
血尿素氮(mmol/L)
对照组	4.83±0.69	5.16±0.21^a	4.87±0.56^a	4.88±0.46^a	4.74±0.48^a
模型组	5.11±0.49	7.52±0.81	13.55±1.99	62.90±16.52	101.03±16.05
外泌体组	4.45±1.06	6.13±1.33	13.77±2.22^a	29.89±6.58^a	52.37±8.38^a
混合囊泡组	5.10±0.79	4.43±1.23^ab	34.75±16.84^a	27.76±11.93^a	35.03±11.18^ab

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