切换至 "中华医学电子期刊资源库"

中华肾病研究电子杂志 ›› 2021, Vol. 10 ›› Issue (02) : 70 -74. doi: 10.3877/cma.j.issn.2095-3216.2021.02.003

所属专题: 文献

论著

双侧肾脏缺血再灌注所致急性肾损伤小鼠行为及情绪障碍的研究
宫铭1, 刘冉1, 刘娇娜1, 吴玲玲1, 陈钰澜1, 王瑾1, 冯哲1, 张利1, 孙雪峰1,(), 陈香美1,()   
  1. 1. 100853 北京,解放军总医院第一医学中心肾脏病医学部,解放军肾脏病研究所,肾脏疾病国家重点实验室,国家慢性肾病临床医学研究中心,肾脏疾病研究北京市重点实验室
  • 收稿日期:2021-01-15 出版日期:2021-04-30
  • 通信作者: 孙雪峰, 陈香美
  • 基金资助:
    国家重点研发计划(2017YFA0103203); 军队后勤科研项目(BLB19J009); 国家自然科学基金重点项目(82030025)

Behavioral and emotional disorders in mice with acute kidney injury induced by bilateral renal ischemia reperfusion

Ming Gong1, Ran Liu1, Jiaona Liu1, Lingling Wu1, Yulan Chen1, Jin Wang1, Zhe Feng1, Li Zhang1, Xuefeng Sun1,(), Xiangmei Chen1,()   

  1. 1. Department of Nephrology, First Medical Center of Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853, China
  • Received:2021-01-15 Published:2021-04-30
  • Corresponding author: Xuefeng Sun, Xiangmei Chen
引用本文:

宫铭, 刘冉, 刘娇娜, 吴玲玲, 陈钰澜, 王瑾, 冯哲, 张利, 孙雪峰, 陈香美. 双侧肾脏缺血再灌注所致急性肾损伤小鼠行为及情绪障碍的研究[J]. 中华肾病研究电子杂志, 2021, 10(02): 70-74.

Ming Gong, Ran Liu, Jiaona Liu, Lingling Wu, Yulan Chen, Jin Wang, Zhe Feng, Li Zhang, Xuefeng Sun, Xiangmei Chen. Behavioral and emotional disorders in mice with acute kidney injury induced by bilateral renal ischemia reperfusion[J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2021, 10(02): 70-74.

目的

探讨急性肾损伤(AKI)小鼠的脑功能障碍表型。

方法

利用C57BL/6J小鼠构建双侧肾脏缺血再灌注模型,观察AKI发生后小鼠在旷场实验、悬尾实验及巴恩斯迷宫实验中的表现变化,以野生小鼠为对照。

结果

与对照组比较,模型组小鼠旷场实验的中心区移动距离百分比明显减少;悬尾实验中运动幅度小于下限的总时长明显增加;巴恩斯迷宫实验测试期的嗅闻区总时间明显减少。

结论

AKI小鼠的记忆功能受损,并伴随焦虑和抑郁的情绪障碍。

Objective

The purpose of this experiment was to explore the phenotype of brain dysfunction in mice with acute kidney injury.

Methods

The model of bilateral renal ischemia-reperfusion was constructed in C57BL/6J mice. Manifestation changes of the mice with acute kidney injury were observed with the open-field test, tail suspension test, and Barnes maze test. The wild type C57BL/6J mice were used as the control.

Results

Compared with the control group, the model group showed significantly less percentage of center-moving distance in the open-field test, significantly longer total-duration when the motion amplitude was less than the lower limit in the tail suspension test, and significantly shorter total-time of the olfactory region in the Barnes maze test.

Conclusion

The mice with acute kidney injury showed impaired memory function, together with mood disorders of anxiety and depression.

图1 小鼠血清尿素氮水平变化情况及肾脏Masson染色结果
图2 小鼠旷场实验结果
图3 小鼠悬尾实验结果
图4 小鼠巴恩斯迷宫实验结果
[1]
Hoste EAJ, Kellum JA, Selby NM, et al. Global epidemiology and outcomes of acute kidney injury [J]. Nat Rev Nephrol, 2018, 14(10): 607-625.
[2]
蔡广研,陈香美. 战创伤及特殊作业环境相关急性肾损伤的防治 [J]. 解放军医学杂志,2019, 44(7): 546-549.
[3]
Lee SA, Cozzi M, Bush EL, et al. Distant organ dysfunction in acute kidney injury: a review [J]. Am J Kidney Dis, 2018, 72(6): 846-856.
[4]
Shiao C, Wu P, Huang T, et al. Long-term remote organ consequences following acute kidney injury [J]. Crit Care, 2015, 19: 438.
[5]
冯哲,陈香美. 急性肾损伤导致远隔器官功能障碍的机制 [J/CD]. 中华肾病研究电子杂志,2015, 4(3): 155-159.
[6]
宫铭,陈香美. 急性肾损伤后脑损伤的研究进展 [J/CD]. 中华肾病研究电子杂志,2021, 10(1): 40-43.
[7]
Tsai H, Yen R, Lin C, et al. Increased risk of dementia in patients hospitalized with acute kidney injury: a nationwide population-based cohort study [J]. PLoS One, 2017, 12(2): e171671.
[8]
Susantitaphong P, Cruz DN, Cerda J, et al. World incidence of AKI: a meta-analysis [J]. Clin J Am Soc Nephrol, 2013, 8(9): 1482-1493.
[9]
Kao CC, Yang WS, Fang JT, et al. Remote organ failure in acute kidney injury [J]. J Formos Med Assoc, 2019, 118(5): 859-866.
[10]
Zhao L, Cao X, Li L, et al. Acute kidney injury sensitizes the brain vasculature to Ang II (angiotensin II) constriction via FGFBP1 (fibroblast growth factor binding protein 1) [J]. Hypertension, 2020, 76(6): 1924-1934.
[11]
Malek M. Brain consequences of acute kidney injury: focusing on the hippocampus [J]. Kidney Res Clin Pract, 2018, 37(4): 315-322.
[12]
Tanaka S, Okusa MD. Crosstalk between the nervous system and the kidney [J]. Kidney Int, 2020, 97(3): 466-476.
[13]
Chou A, Lee C, Chen C, et al. Hippocampal transcriptional dysregulation after renal ischemia and reperfusion [J]. Brain Res, 2014, 1582: 197-210.
[14]
Firouzjaei MA, Haghani M, Shid Moosavi SM. Renal ischemia/reperfusion induced learning and memory deficit in the rat: insights into underlying molecular and cellular mechanisms [J]. Brain Res, 2019, 1719: 263-273.
[15]
Kovalcíková A, Gyurászová M, Vavrincová-Yaghi D, et al. Oxidative stress in the brain caused by acute kidney injury [J]. Metab Brain Dis, 2018, 33(3): 961-967.
[16]
Yu F, Liang H, Xin S. Renal ischemia reperfusion cause brain hippocampus oxidative damage and inhibition effect [J]. Afr J Tradit Complement Altern Med, 2016, 13(5): 61-66.
[17]
Rosenfeld CS, Ferguson SA. Barnes maze testing strategies with small and large rodent models [J]. J Vis Exp, 2014, 84: e51194.
[18]
Pitts MW. Barnes maze procedure for spatial learning and memory in mice [J]. Bio Protoc, 2018, 8(5): e2744.
[19]
Gawel K, Gibula E, Marszalek-Grabska M, et al. Assessment of spatial learning and memory in the Barnes maze task in rodents-methodological consideration [J]. Naunyn Schmiedebergs Arch Pharmacol, 2019, 392(1): 1-18.
[20]
Franca M, Ramos R, Oliveira WH, et al. Tadalafil restores long-term memory and synaptic plasticity in mice with hepatic encephalopathy [J]. Toxicol Appl Pharmacol, 2019, 379: 114673.
[21]
Tahamtan M, Moosavi SMS, Sheibani V, et al. Erythropoietin attenuates motor impairments induced by bilateral renal ischemia/reperfusion in rats [J]. Fundam Clin Pharmacol, 2016, 30(6): 502-510.
[22]
Seibenhener ML, Wooten MC. Use of the open field maze to measure locomotor and anxiety-like behavior in mice [J]. J Vis Exp, 2015, 96: e52434.
[23]
Kuniishi H, Ichisaka S, Yamamoto M, et al. Early deprivation increases high-leaning behavior, a novel anxiety-like behavior, in the open field test in rats [J]. Neurosci Res, 2017, 123: 27-35.
[24]
Gao N, Zheng W, Murezati T, et al. GW117: a novel serotonin (5-HT2C) receptor antagonist and melatonin (MT1/MT2) receptor agonist with potential antidepressant-like activity in rodents [J]. CNS Neurosci Ther, 2021, Epub ahead of print.
[25]
Can A, Dao DT, Terrillion CE, et al. The tail suspension test [J]. J Vis Exp, 2012, 59: e3769.
[1] 韩圣瑾, 周正武, 翁云龙, 黄鑫. 碳酸氢钠林格液联合连续性肾脏替代疗法对创伤合并急性肾损伤患者炎症水平及肾功能的影响[J]. 中华危重症医学杂志(电子版), 2023, 16(05): 376-381.
[2] 张秋彬, 张楠, 林清婷, 徐军, 朱华栋, 姜辉. 急性胰腺炎合并急性肾损伤患者的预后评估[J]. 中华危重症医学杂志(电子版), 2023, 16(05): 382-389.
[3] 莫小乔, 胡喆莹, 廖冬花, 谢天. 脓毒症继发急性肾损伤患者死亡风险预测模型构建及评估[J]. 中华危重症医学杂志(电子版), 2023, 16(03): 198-206.
[4] 吴庆华, 冒勇, 闫效坤. AECOPD并发AKI的危险因素分析[J]. 中华肺部疾病杂志(电子版), 2023, 16(04): 529-531.
[5] 李青霖, 宋仁杰, 周飞虎. 一种重型劳力性热射病相关急性肾损伤小鼠模型的建立与探讨[J]. 中华肾病研究电子杂志, 2023, 12(05): 265-270.
[6] 任加发, 邬步云, 邢昌赢, 毛慧娟. 2022年急性肾损伤领域基础与临床研究进展[J]. 中华肾病研究电子杂志, 2023, 12(05): 276-281.
[7] 李金璞, 饶向荣. 抗病毒药物和急性肾损伤[J]. 中华肾病研究电子杂志, 2023, 12(05): 287-290.
[8] 程庆砾. 新冠病毒感染与肾脏[J]. 中华肾病研究电子杂志, 2023, 12(04): 240-240.
[9] 宋艳琪, 任雪景, 王文娟, 韩秋霞, 续玥, 庄凯婷, 肖拓, 蔡广研. 间充质干细胞对顺铂诱导的小鼠急性肾损伤中细胞铁死亡的作用[J]. 中华肾病研究电子杂志, 2023, 12(04): 187-193.
[10] 苗软昕, 乔晞. Toll样受体在脓毒症性急性肾损伤中的作用[J]. 中华肾病研究电子杂志, 2023, 12(04): 210-214.
[11] 李娜, 朱国贞. 肠道菌群及其代谢产物在急性肾损伤中的作用研究进展[J]. 中华肾病研究电子杂志, 2023, 12(04): 215-219.
[12] 杨长沅, 凌曦淘, 丘伽美, 段若兰, 李琴, 林玉婕, 秦新东, 侯海晶, 卢富华, 苏国彬. 慢性肾脏病患者衰弱的筛查/评估工具研究进展[J]. 中华肾病研究电子杂志, 2023, 12(04): 229-233.
[13] 于天宇, 杨悦, 陆海涛, 田志永, 李文歌. 高龄急性肾损伤患者连续性肾脏替代治疗的预后及影响因素[J]. 中华肾病研究电子杂志, 2023, 12(03): 134-138.
[14] 吴蓉菊, 向平超. COPD频繁急性加重表型与炎性因子相关性研究[J]. 中华临床医师杂志(电子版), 2023, 17(9): 939-947.
[15] 易成, 韦伟, 赵宇亮. 急性肾脏病的概念沿革[J]. 中华临床医师杂志(电子版), 2023, 17(08): 906-910.
阅读次数
全文


摘要