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中华肾病研究电子杂志 ›› 2022, Vol. 11 ›› Issue (04) : 219 -223. doi: 10.3877/cma.j.issn.2095-3216.2022.04.007

综述

免疫电镜技术在肾脏疾病诊断和研究中的应用
沈婉君1, 王田田1, 尹智炜2, 谢院生1,()   
  1. 1. 100853 北京,解放军总医院第一医学中心肾脏病医学部、解放军肾脏病研究所、肾脏疾病国家重点实验室、国家慢性肾病临床医学研究中心、肾脏疾病研究北京市重点实验室
    2. 100853 北京,解放军总医院第一医学中心肾脏病医学部、解放军肾脏病研究所、肾脏疾病国家重点实验室、国家慢性肾病临床医学研究中心、肾脏疾病研究北京市重点实验室;050017 石家庄,河北医科大学中西医结合学院
  • 收稿日期:2022-03-13 出版日期:2022-08-28
  • 通信作者: 谢院生
  • 基金资助:
    国家自然科学基金面上项目(82174115)

Application of immunoelectron microscopy in diagnosis and research of renal diseases

Wanjun Shen1, Tiantian Wang1, Zhiwei Yin2, Yuansheng Xie1,()   

  1. 1. Department of Nephrology, First Medical Center of Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853
    2. Department of Nephrology, First Medical Center of Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853; College of Integrated Chinese and Western Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei Province; China
  • Received:2022-03-13 Published:2022-08-28
  • Corresponding author: Yuansheng Xie
引用本文:

沈婉君, 王田田, 尹智炜, 谢院生. 免疫电镜技术在肾脏疾病诊断和研究中的应用[J/OL]. 中华肾病研究电子杂志, 2022, 11(04): 219-223.

Wanjun Shen, Tiantian Wang, Zhiwei Yin, Yuansheng Xie. Application of immunoelectron microscopy in diagnosis and research of renal diseases[J/OL]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2022, 11(04): 219-223.

免疫电镜技术是抗原抗体反应和电子显微镜相结合的技术,可在亚细胞和超微结构水平对抗原进行定位观察分析。其在肾脏疾病诊断和研究领域的应用包括单克隆免疫球蛋白沉积性肾病、免疫复合物介导的肾小球疾病、肾小球结构研究、肾小管疾病发病机制以及其他肾脏疾病。本文较为全面地展示了免疫电镜技术在肾脏疾病诊断和研究领域的重要作用和发展趋势,以期对今后相关领域的研究有所启发。

Immunoelectron microscopy is a combination of antigen-antibody reaction and electron microscopy, which can be used to observe and analyze antigens at subcellular and ultrastructural level. Its applications in diagnosis and research of renal diseases involve monoclonal immunoglobulin deposition nephropathy, immune complex-mediated glomerular diseases, glomerular structure research, pathogenesis of renal tubular diseases, and other renal diseases. This review comprehensively showed the important role and development trend of immunoelectron microscopy in diagnosis and research of renal diseases, in order to support the future research in related fields.

图1 免疫电镜技术的应用场景、拓展应用及优劣势
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