对小鼠肾脏透明荧光标本制备及光片荧光显微镜成像技能的教学进行分析。

参训学员为2例博士研究生和2例其他科研人员，培训教师为资深肾脏生物成像技师，培训时长31 d；在培训不同时间点对小鼠的荧光抗体注射成功率、术前死亡率、心脏灌注出血率、肾脏取材成功率、肾脏透明程度、肾脏荧光成像效果等进行评估和统计学分析。

与培训第1天相比，培训第5天的荧光抗体注射成功率和肾脏取材成功率较高，而小鼠术前死亡率和心脏灌注出血率降低(P均<0.01)。培训第10、20和30天的肾脏透明程度逐步提升，而且第30天的小鼠肾脏透明效果达到标准；培训第31天，参训学员的光片荧光显微镜成像效果也达到标准。

对8项实验操作技能和5个教学要点进行的31 d解析教学，使参训学员掌握了小鼠肾脏的透明荧光标本制备和光片荧光显微镜成像技能。

To analyze the teaching of mouse kidney transparent fluorescent specimen preparation and light-sheet fluorescence microscopy imaging skills.

The participants included 2 doctoral students and 2 other scientific researchers, and the training instructor was a senior renal biological imaging technician. The training lasted for 31 days. At different time points during the training, the success rate of fluorescent antibody injection in mice, preoperative mortality rate, cardiac perfusion bleeding rate, success rate of kidney sampling, kidney transparency degree, and kidney fluorescence imaging effect were evaluated and statistically analyzed.

Compared with those of the first day of training, the success rates of fluorescent antibody injection and kidney harvesting were higher on the fifth day of training, while the preoperative mortality rate of mice and the bleeding rate during cardiac perfusion were lower (all P<0.01). On the 10th, 20th, and 30th days of training, the transparency of the kidneys gradually improved. And on the 30th day, the transparency of the mouse kidneys met the standard. On the 31st day of training, the imaging effect of the light-sheet fluorescence microscopy for the participating students also met the standard.

A 31-day analytical teaching program covering 8 experimental operation skills and 5 teaching essentials enabled the trainees to master the preparation of transparent fluorescent specimens of mouse kidneys and the imaging skills of light-sheet fluorescence microscopy.